ARID3A monoclonal antibody (M01), clone 1A11
产品名称： ARID3A monoclonal antibody (M01), clone 1A11
英文名称： ARID3A monoclonal antibody (M01), clone 1A11
产品编号： H00001820-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant ARID3A.
- Immunogen:
- ARID3A (NP_005215, 317 a.a. ~ 416 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- QYMKYLYPYECEKRGLSNPNELQAAIDSNRREGRRQSFGGSLFAYSPGGAHGMLSSPKLPVSSLGLAASTNGSSITPAPKIKKEEDSAIPITVPGRLPVS
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry.
Kang UB, Yeom J, Kim HJ, Kim H, Lee C.J Proteomics. 2011 Nov 26.
- Applications
- Western Blot (Cell lysate)
- ARID3A monoclonal antibody (M01), clone 1A11 Western Blot analysis of ARID3A expression in K-562 ( Cat # L009V1 ).
- Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged ARID3A is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi ( Cat # H00001820-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody (M01), clone 1A11 (Cat # H00001820-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 1820
- GeneBank Accession#:
- NM_005224
- Protein Accession#:
- NP_005215
- Gene Name:
- ARID3A
- Gene Alias:
- BRIGHT,DRIL1,DRIL3,E2FBP1
- Gene Description:
- AT rich interactive domain 3A (BRIGHT-like)
- Omim ID:
- 603265
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq
- Other Designations:
- ARID domain-containing 3A,AT rich interactive domain 3A (BRIGHT- like),AT rich interactive domain 3A (BRIGHT- like) protein,B-cell regulator of IgH transcription,E2F-binding protein 1,dead ringer-like 1
- Related Disease